We have just published a paper in Nature Communications, MINFLUX microscopy resolves subunits of the cardiac ryanodine receptor and its 3D orientation in cells.

MINFLUX microscopy resolves subunits of the cardiac ryanodine receptor

We present purely optical data of RyR2 distribution with sub-molecular resolution by applying 3D MINFLUX microscopy. Using single-domain antibodies and DNA-PAINT we determine the location of individual RyR2 subunits with high precision (~3 nm) and resolve the 3D orientations of RyR2s in-situ. We measured labeling efficiencies of ~50%, implying RyR2 tetramer detection probability approaching 95%. Ventricular myocytes from mice contained large clusters containing many tens of close-packed RyR2s, resolving apparent discrepancies between electron microscopy and previous super-resolution microscopy data.

For details check out the paper.